Product details
| Source | HEK293 Cells |
| Species | Human |
| Bio-activity | 1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is > 1,000 pmoles/min/µg. (Activation description: The proenzyme needs to be activated by APMA for an activated form) 2. Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc, the EC50 of Human TIMP2 hFc is 6.0-30.0 ng/mL. |
| Length | A DNA sequence encoding the native human MMP2 (NP_004521.1) (Ser 2-Cys 660) was expressed and purified. |
| Molecular Mass | The recombinant human MMP2 consists of 631 amino acids and migrates as an 72 kDa band as predicted in SDS-PAGE under reducing conditions. |
| Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | ＜1.0 EU per μg by the LAL method. |
| Buffer | Lyophilized from sterile 0.05 % Brij-35, 150 mM NaCl, 5 mM CaCl2, 50 mM Tris, pH 7.5. |
| Storage | Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |