Product details

Intended Use
Picro-Sirius Red Stain Kit is intended for use in the histological visualization of thin septa and collagen fibers. This modification of the Picro-Sirius Red stain eliminates the yellow cytoplasmic staining that can obscure thin collagenous septa. Using this procedure easily allows viewing of collagenous septa as thin as 0.2-0.5 microns. The PSR stain may be viewed using standard light microscopy or polarized light resulting in birefringence of the collagen fibers.
Light Microscopy
Collagen: Red
Septa: Red
Cytoplasm: Colorless to Slightly Yellow
Polarized Light Microscopy
Collagen: Yellow-Orange and Green Birefringence
Control Tissue: Cardiac Muscle Uterus Lung Kidney.
Sample Type
Tissue sections
Kit Contents
Acetic Acid Solution, 1 x 250ml Phosphomolybdic Acid Solution (0.2%), 1 x 250ml Picro Sirius Red Solution, 1 x 250ml
Storage
RT

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