Mouse Adiponectin ELISA Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes mouse Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Serum, Plasma, Cell Culture Supernates, Tissue homogenates, cell lysates

Mouse

1. Microplate - antibody coated 96 - well Microplate (8 well × 12 strips) 1 plate
2. Protein standard - 4000 pg/bottle; lyophilized* 2 bottles
3. Detection antibody, biotinylated (100X) - 120 µL/vial 1 vial
4. Streptavidin-HRP (100X) - 120 µL/vial 1 vial
5. Sample Diluent PT 1 - 30 mL/bottle. For serum and plasma samples and tissue homogenates. 2 bottles
6. Sample Diluent PT 1-ef - 30 mL/bottle. For cell culture supernatants 1 bottle
7. Detection Diluent - 30 mL/bottle 1 bottle
8. Wash Buffer Concentrate (20X) - 30 mL/bottle 1 bottle
9. Tetramethylbenzidine Substrate (TMB) - 12 mL/bottle 1 bottle
10. Stop Solution - 12 mL/bottle 1 bottle
11. Plate Cover Seals 3 pieces

31.25-2000 pg/mL

6.9 pg/mL