Product details
| Intended Use | Human Orexin (OX) ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of orexin in human serum, plasma, tissue homogenates and other biological fluids.Human Orexin (OX) ELISA Kit |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Species | Human |
| Detection Method | Competitive Inhibition |
| Kit Contents | Assay plate (96 Wells): 1 Standard: 2 Detection Reagent A: 1 Detection Reagent B: 1×120μL Reagent Diluent: 1×300μL TMB Substrate: 1×9mL Wash Buffer (30 ×): 1×20mL Standard Diluent: 1×20mL Assay Diluent A: 1×12mL Assay Diluent B 1×12mL Stop Solution 1×6mL Plate sealer for 96 wells: 4 |
| Range | 12.35-1,000pg/mL |
| Sensitivity | ≤5.15pg/mL |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Orexin (OX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Orexin (OX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |