Product details
| Intended Use | This assay has high sensitivity and excellent specificity for detection of Human VD. No significant cross-reactivity or interference between Human VD and analogues was observed. |
| Principle | This assay employs a two-site sandwich ELISA to quantitate VD in samples. An antibody specific for VD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VD bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Sample Type | Serum, Plasma, Other biological fluids |
| Species | Human (Homo sapiens) |
| Detection Method | Sandwich |
| Kit Contents | Assay plate (96 Wells): 1 Standard (lyophilized): 2 Sample Diluent: 1 x 20 mL HRP-Conjugate: 1 x 60 μL HRP-Conjugate Diluent: 1 x 10 mL Wash Buffer (concentrate 25 x): 1 x 20 mL Substrate Solution: 1 x 12 mL Stop Solution: 1 x 10 mL Adhesive Films: 4 |
| Storage | 4℃ |
| Range | 6.25-400 pg/mL |
| Sensitivity | 3.12 pg/mL |
| Precision | Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |