Product details
| Intended Use | This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and
other biological fluids. |
| Principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with
an antibody specific to Human IFN-γ. Samples (or Standards) are added to the micro ELISA plate wells and combined
with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish
Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed
away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection
antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the
addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a
wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the
concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve. |
| Species | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant or other biological fluids |
| Kit Contents | Micro ELISA Plate (Dismountable): 8 wells ×12 strips Reference Standard: 2 vials Concentrated Biotinylated Detection Ab (100×): 1 vial, 120 μL Concentrated HRP Conjugate (100×): 1 vial, 120 μL Reference Standard & Sample Diluent: 1 vial, 20mL Biotinylated Detection Ab Diluent: 1 vial, 14mL HRP Conjugate Diluent: 1 vial, 14mL Concentrated Wash Buffer (25×): 1 vial, 30mL Substrate Reagent: 1 vial, 10mL Stop Solution: 1 vial, 10mL Plate Sealer: 5 pieces |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 9.38 pg/mL |