Product details
| Product Overview | Recombinant PCR grade Proteinase K is expressed in Pichia pastoris. |
| Description | The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. The specifications of the recombinant enzyme are the same as those of the native protease. The amino acid sequence (molecular weight) and the molecule structure are identical. However, the recombinant preparation is much purer than the native enzyme, as it is DNA-free, according to the current quality control procedures, and is therefore well suited for isolating PCR and RT-PCR templates. |
| Source | Pichia pastoris |
| Form | Liquid |
| Bio-activity | ~2.5 units/mg protein (Approximately 2.5 U/mg (Chromozym assay); ≥30 U/mg hemoglobin assay. Volume Activity: Approximately 50 U/ml solution (chromozyme assay); approximately 600 U/ml solution (hemoglobin assay). One unit is the enzyme activity which cleaves at +25 °C in 1 min 18 mmol Chromozym TRY (equivalent to 600 U/ml with the hemoglobin assay). Refer to the Certificate of Analysis for specific values for the present lot. |
| Applications | This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. |
| Buffer | Buffered aqueous solution (18 ± mg/mL; pH 7.5) |
| Storage | Store at 2-8℃. |
| Notes | This preparation is free of RNases, DNases, and DNA, according to the current quality control procedures. |